ULTRAVIOLET LIGHT INDUCED MUTATION AND DEOXYRIBONUCLEIC ACID REPLICATION IN BACTERIA

Ultraviolet Light Induced Mutation and Deoxyribonucleic Acid Replication in Bacteria.

HE terminal process through which the genetic change induced by ultraviolet Tlight (UV) is incorporated into the genetic apparatus is the initial postirradiation deoxyribonucleic acid (DNA) replication ( HAAS and DOUDNEY 1959; DOUDNEY and HAAS 1960). It has been suggested that two discrete radiation effects are prerequisite to incorporation of the “potential mutation” into DNA ( DOUDNEY, KADA a...

Ultraviolet light-induced mutation and deoxyribonucleic acid synthesis in Escherichia coli.

Exposure of a bacterial population to a mutagenic dose of ultraviolet light generally does not establish phenotypically mutant cells immediately. Rather, a period of subeultivation of the irradiated cells is required before newly induced mutants can be detected (Demeree, 1946). It has been postulated (see Witkin, 1956, for a recent discussion) that the delay between irradiation and phenotypic e...

Ultraviolet light alteration of cellular deoxyribonucleic acid in vivo.

DNA after irradiation with ultraviolet light was immunogenic in rabbits and elicited serum antibodies reacting specifically with UV-irradiated DNA. The serological reactions were demonstrated by immunodiffusion, complement fixation, and immunofluorescence. By immunofluorescence, antisera reacted with cell nuclei of irradiated tissue sections but not with unirradiated tissue. This method was emp...

Presence of an endonuclease specific for ultraviolet light-irradiated deoxyribonucleic acid in a ultraviolet light-sensitive mouse cell mutant Q31.

Deoxyribonucleic acid-deoxyribonucleic acid hybridization assay for replication origin deoxyribonucleic acid of Escherichia coli.

Deoxyribonucleic acid (DNA)-DNA hybridization on nitrocellulose filters can be used to assay for replication origin DNA from Escherichia coli if the DNA attached to the filters is enriched for the replication origin sequences. Such DNA can be readily isolated from very rapidly growing cells. When low amounts of this DNA were attached to filters, radioactively labeled DNA from the replication or...